Identification of Macromolecule

Macromolecules invert more than one molecules. They argon usually imbed in foods. For example, lipids, nucleic acids, carbohydrates and proteins are the living organisms that macromolecules are commonly found. Their functions are in the main storing energy and information. By performing this lab, types of macromolecules are found. There are three different experiments that we performed. They are unity test, Biuret Test and Benedicts test. With 11 upshots includes one terra incognita solution, the macromolecules willing be found in the solutions and also, the mystic solution will be identified after the experiments. In this case, unknown solution is unknown number For example, from Iodine test, if the solution contains glycogen molecule, dis annotate yellow unity solution turns red-brown. If the solution contains starch molecule, the solution will turn blue black colour. For the Benedicts test, blue dark-skinned solution means reducing sugars and coloured precipitate mean s they contain some macromolecules. The colours they represent are yellow, green, red and brown. For the Biuret Test, the colour miscellany occurs when there is protein inside the solution. Colour change will be violet or yellow.Materials and Methods Materials we used were 12 small disposable plastic beakers Pasteur pipettes & bulbs 1 spot-plate test-subway system rack 12 test-tubes 1 test-tube clamp 1 vaultper feeding bottle containing 1% papal bull sulfate 1 dropper bottle containing iodine solution 1 large beaker with boiling chips 1 marker 15 ml of 1% glucose solution 15ml of 0.3% glucose-1-phosphate 15ml of 1% maltose solution 15ml of edulcorate solution 15ml of 1% sucrose solution 15ml of 1% lactose solution 15ml of 1% glycogen solution 15ml of 1% starch solution 15ml of protein, 15ml of beer 15ml of distilled urine supply and 15ml of 210 unknown solution 10% Sodium hydroxide 1% copper sulfate.Methods Three experiments were performed in order, Iodine test, Benedicts t est and Biuret Test. sooner the experiments are begun, put all the 15ml of solutions into the beaker 1 to 12. These beakers are numbered with marker. Tube 1 is modify to with the approximately 1 ml of the liquid from beaker 1 (1/2 of the full Pasteur pipette is approximately 1 ml). Tube 2 is filled with the approximately 1 ml of the liquid from beaker 2. Untill all 12 tubes are filled up with 1 ml of the appropriate solution. 2ml of Benedicts solution is added to each tube. The contents are mixed to each tube thoroughly. Boil the contents of each tube by placing the tube in a boiling water bath which has boiling chips in the water for about 5 minutes and note the colour change. Finally. entirely of the observations are enter and explained. Firstly, for the Iodine test, 1 drop of the solution is put from the beaker 1 into a clean well on a spot-plate. Then one drop of iodine solution is added to the sample drop. This is how iodine test is done. Any colour change is recorded, as we ll as any differences and other observations. Iodine test is repeated on every other remaining solutions. All the observations are recorded on a piece of paper. Benedicts test was done with five procedures. Firstly, the tubes were Observation before and after colour, persuade or negat, beaker and solu name Discussion explaineverythign in detail screw propeller